Overview

  • Product nameAnti-CEBP gamma antibody
    See all CEBP gamma primary antibodies
  • Description
    Mouse monoclonal to CEBP gamma
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-151 of Human CEBP gamma

Properties

Applications

Our Abpromise guarantee covers the use of ab54899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionTranscription factor that binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene. Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites.
  • Sequence similaritiesBelongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C/EBP gamma antibody
    • CCAAT/enhancer binding protein (C/EBP) gamma antibody
    • CCAAT/enhancer binding protein gamma antibody
    • CCAAT/enhancer-binding protein gamma antibody
    • CEBPG antibody
    • CEBPG_HUMAN antibody
    • GPE1BP antibody
    • IG/EBP 1 antibody
    • IG/EBP1 antibody
    see all

Anti-CEBP gamma antibody images

  • All lanes : Anti-CEBP gamma antibody (ab54899) at 1/1000 dilution

    Lane 1 : Mouse adipocyte lysate - untreated
    Lane 2 : Mouse adipocyte lysate - 5nm rapamycin treatment for 48 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated anti-mouse IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 47 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Kun Dong

    See Abreview

  • Western blot against tagged recombinant protein immunogen using ab54899 CEBP gamma antibody at 1ug/ml. Predicted band size of immunogen is 43 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

References for Anti-CEBP gamma antibody (ab54899)

ab54899 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Denaturing (12.5%)
Sample Mouse Cell lysate - other (adipocyte)
Specification adipocyte
Treatment 5nm rapamycin for 48 hours
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. KUN DONG

Verified customer

Submitted Jan 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"