Recombinant
RabMAb

Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)

Overview

  • Product name
    Anti-CECR5 antibody [EPR14793(B)] - C-terminal
    See all CECR5 primary antibodies
  • Description
    Rabbit monoclonal [EPR14793(B)] to CECR5 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CECR5 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BXW7

  • Positive control
    • HeLa, Jurkat, HepG2, U87-MG cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR14793(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 46 kDa).
ICC/IF 1/100.
IP 1/30 - 1/50.

Target

  • Tissue specificity
    Widely expressed.
  • Information by UniProt
  • Database links
  • Alternative names
    • A930002G03Rik antibody
    • Cat eye syndrome chromosome region, candidate 5 antibody
    • Cat eye syndrome chromosome region, candidate 5 homolog antibody
    • Cat eye syndrome critical region protein 5 antibody
    • Cecr5 antibody
    • CECR5_HUMAN antibody
    • MGC25951 antibody
    • OTTMUSP00000025905 antibody
    see all

Images

  • All lanes : Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 46 kDa

  • All lanes : Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 46 kDa

  • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling CECR5 with ab184185. Right lane shows PBS negative control.



    All lanes :

    All lanes :
  • Immunofluorescent analysis of HeLa cells labeling CECR5 with ab184185 at a 1/100 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

References

ab184185 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184185.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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