Overview

  • Product name
    Anti-Cecropin B antibody
  • Description
    Rabbit polyclonal to Cecropin B
  • Specificity
    ab27571 recognises cecropin B. The antibody may also recognize naturally occurring cecropin A.
  • Tested applications
    Suitable for: ELISA, IP, WBmore details
  • Species reactivity
    Reacts with: Silk worm
  • Immunogen

    Synthetic peptide: KWKVFKKIEK VGRNIRNGIV KAGPAIAVLG EAKAL, corresponding to amino acids 27-61 of Cecropin B modified at aa 37 from the sequence which is found in silkworm.

  • General notes


    Anti-cecropin B is useful for the detection of cecropin B in various immunological assays.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.42% Potassium phosphate, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using cecropin B coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified cecropin B. Reactivity against other forms of cecropins has not yet been determined.
  • Primary antibody notes
    Anti-cecropin B is useful for the detection of cecropin B in various immunological assays.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB 1/1000. Detects a band of approximately 4.4 kDa (predicted molecular weight: 7 kDa).

Target

  • Relevance
    Cecropins are produced by insects, particularly under conditions of infection. Cecropins are bioactive peptides that exhibit activities by interacting with membranes and forming transmembrane channels that allow the free flow of electrolytes, metabolites and water across the phospholipid bilayers. Cecropins A, B and D are close homologues consisting of 35-39 residues. They are found in the pupae of the cecropin moth, but related homologues named lepodopteran, bactericidin, moricin and sarcotoxin are produced by other insects.
  • Cellular localization
    Secreted
  • Database links
    • Alternative names
      • CECB1 antibody
      • CECB2 antibody
      • Lepidopteran A/B antibody

    References for Anti-Cecropin B antibody (ab27571)

    This product has been referenced in:
    • Suzuki J  et al. Symbiosis with Francisella tularensis provides resistance to pathogens in the silkworm. Sci Rep 6:31476 (2016). WB . Read more (PubMed: 27507264) »
    • Dandekar AM  et al. An engineered innate immune defense protects grapevines from Pierce disease. Proc Natl Acad Sci U S A 109:3721-5 (2012). Read more (PubMed: 22355130) »

    See all 2 Publications for this product

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    Thank you for your inquiry.

    We do not have confirming data for possible reactivity of this antibody with Cecropin A. The statement was based on sequence homology only.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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