The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested in peptide ELISA at 1:64000.
IHC-P: Use at a concentration of 5 µg/ml.
Western Blot: Preliminary experiments gave an approx 35kDa band in K562 lysates at 1µg/ml. However, this was accompanied by stronger bands at approx 45kDa and 48kDa. Please note that currently we cannot find an explanation in the literature for these extra bands given the predicted size of approx. 35kDda according to NP_057399.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May function as an adapter protein. Required for efficient phagocytosis of apoptotic cells. Modulates cellular glycosphingolipid and cholesterol transport. May play a role in the internalization and endosomal trafficking of various LRP1 ligands, such as PSAP. Increases cellular levels of GTP-bound ARF6.
Widely expressed. Detected in macrophages, pancreas, kidney, skeletal muscle, heart, colon, intestine, lung, placenta and ovary.
Belongs to the ced-6 family. Contains 1 PID domain.
Cytoplasm. May associate with the cytoplasmic side of the plasma membrane and early endosomes.
ab2751 at 5ug/ml staining CED6 in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.