Overview

  • Product name
    Anti-CEE antibody
  • Description
    Rabbit polyclonal to CEE
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 277-327 of Human CEE (NP_057033.2)

  • Positive control
    • Whole cell lysate from HeLa and 293T cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2 - 5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 37 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting.
    • Sequence similarities
      Belongs to the GET4 family.
    • Cellular localization
      Cytoplasm > cytosol.
    • Information by UniProt
    • Database links
    • Alternative names
      • C7orf20 antibody
      • CGI 20 antibody
      • CGI20 antibody
      • Conserved edge expressed protein antibody
      • Conserved edge protein antibody
      • Conserved edge-expressed protein antibody
      • GET 4 antibody
      • GET4 antibody
      • GET4_HUMAN antibody
      • Golgi to ER traffic protein 4 homolog (S. cerevisiae) antibody
      • Golgi to ER traffic protein 4 homolog antibody
      • Transmembrane domain recognition complex 35 kDa subunit antibody
      • Transmembrane domain recognition complex 35kDa antibody
      • TRC35 antibody
      • UPF0363 protein C7orf20 antibody
      see all

    Images

    • All lanes : Anti-CEE antibody (ab93801) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 37 kDa


      Exposure time : 3 minutes
    • Detection of CEE in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93801 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

    References

    ab93801 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab93801.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up