Cell Cycle Assay Kit (Fluorometric - Red) (ab112117)

Overview

  • Product nameCell Cycle Assay Kit (Fluorometric - Red)
    See all Cell Cycle kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeCell-based (quantitative)
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab112117 is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Red CCS1 in fixed cells. The dye passes through a permeabilized membrane and intercalates into cellular DNA. The signal intensity of Nuclear Red CCS1 is directly proportional to DNA content after RNA is degraded by RNase provided in the kit. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be monitored with a flow cytometer at Ex/Em = 490/620 nm (FL2 channel).

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  • Notes

    Store ab112117 desiccated.

  • Tested applicationsSuitable for: Flow Cyt, Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab112117 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.
    Functional Studies Use at an assay dependent concentration.

    Cell Cycle Assay Kit (Fluorometric - Red) images

    • DNA profile in growing Jurkat cells.
      Jurkat cells were dye-loaded with Red Fluorescence Probe and RNase A for 30 minutes. The fluorescence intensity of Red Fluorescence Probe was measured with a flow cytometer, using the FL2 channel.

    Protocols

    References for Cell Cycle Assay Kit (Fluorometric - Red) (ab112117)

    ab112117 has not yet been referenced specifically in any publications.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"