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ab112117 is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Red CCS1 in fixed cells. The dye passes through a permeabilized membrane and intercalates into cellular DNA. The signal intensity of Nuclear Red CCS1 is directly proportional to DNA content after RNA is degraded by RNase provided in the kit. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be monitored with a flow cytometer at Ex/Em = 490/620 nm (FL2 channel).
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Store ab112117 desiccated.
|100X RNase A||1 x 250µl|
|Assay Buffer||1 x 50ml|
|Nuclear Red CCS1||1 x 250µl|
Our Abpromise guarantee covers the use of ab112117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
ab112117 has not yet been referenced specifically in any publications.