Monitoring cell cytotoxicity is one of the most essential tasks for studying cellular functions. There are a variety of parameters that can be used. ab112118 uses a proprietary water-soluble dye that changes its absorption spectra upon cellular reduction. The absorption ratio change is directly proportional to the number of living cells. The characteristics of its high sensitivity, non-radioactivity and no-wash method make ab112118 suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds.
ab112118 does not require pre-mixing of components and has higher sensitivity compared to tetrazolium based colorimetric assays (such as MTT and XTT). It comes with reagents sufficient to run 1000 assays. The kit components are quite stable with minimal cytotoxicity, thus longer incubation times (such as 24-48 hours) are possible if required.
ab112118 is robust and convenient to use. It can be readily adapted for a wide variety of instrument platforms. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
Visit our FAQs page for tips and troubleshooting.
ab112118 should be stored desiccated.
As low as 300 cells can be accurately quantified using ab112118.
|Assay Solution||Component A||1 x 20ml|
Our Abpromise guarantee covers the use of ab112118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
•HeLa: day before experiment cells seeded in 96 wp @ 1e4 cells/well and treated with 1µM, 10 µM and 50 µM Camptothecin (incubated for 24h); on the day of experiment cells incubated with Assay Solution for 2 hours.
•NIH3T3: day before experiment cells seeded in 96 wp @ 1e4 cells/well and treated with 1µM, 10 µM and 50 µM Camptothecin (incubated for 24h); on the day of experiment cells incubated with Assay Solution for 2 hours.
ab112118 has not yet been referenced specifically in any publications.