Overview

  • Product nameCell Cytotoxicity Assay Kit (Fluorometric)
    See all Cell Cytotoxicity kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Human, Chinese Hamster
    Predicted to work with: all Mammals
  • Product overview

    The measurement of mitochondrial dehydrogenases (e.g. LDH) activity is a well-accepted assay to quantify cell numbers and monitor cell viability.
    ab112119 Cell Cytotoxicity Assay Kit provides a fast, simple, accurate and homogeneous assay for the fluorimetric detection of viable cells. ab112119 is based on the observation that oxidized non-fluorescent blue resazurin is reduced to a red fluorescent dye (resorufin) by accepting an electron from mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells.

    ab112119 is more sensitive for cell proliferation and cytotoxicity than other assays such as MTT. The kit components are quite stable with minimal cytotoxicity, thus a longer incubation time (such as 24-48 hours) is possible if required.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    ab112119 should be stored desiccated

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab112119 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Cell Cytotoxicity Assay Kit (Fluorometric) images

    • CHO-K1 cell number response was measured with ab112119. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 20 µL/well of Assay Solution for 3 hours at 37 °C. The absorbance intensity was measured at 570 nm and 605 nm. The fluorescence intensity was linear (R2 = 0.998) to the cell number as indicated. The detection limit from the blank controls was 60 cells/well (n=6).

    Protocols

    References for Cell Cytotoxicity Assay Kit (Fluorometric) (ab112119)

    ab112119 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"