MitoSciences (MS862)

Cell Fractionation Kit - High Throughput (HT) (ab109718)

Overview

  • Product nameCell Fractionation Kit - High Throughput (HT)
    See all Cell Fractionation kits
  • Tests
    1 x 96 well plate
  • Assay typeDirect
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Human, a wide range of other species
  • Product overview

    Abcam's Cell Fractionation Kit - High Throughput (HT), provides a method and reagents for a simple and rapid preparation of cytosolic, mitochondrial and nuclear fractions from adherent cells.

    The Cell Fractionation Kit is designed to produce highly enriched fractions that allow for the monitoring of proteins of interest through various cellular compartments. It is not designed to produce purified cellular fractions.

    The kit is especially suitable for, but not limited to, high throughput fractionation of adherent cells in a 96-well plate format. The entire fractionation of 96 samples into the cytosolic, mitochondrial and nuclear fraction is achieved in one hour. The kit is based on sequential detergent extraction of cytosolic, mitochondrial and nuclear proteins without the need for mechanical disruption of cells, and thus fractionates adherent cells into cytosol-containing, mitochondria-containing and nuclei-containing fractions. These fractions are referred throughout the protocol as cytosolic, mitochondrial and nuclear fractions. The kit prepares sufficient sample material for subsequent Western blot analyses, dot blot analyses or for analyses by microplate ELISA assays.

     

    ab109718 is designed to allow the measurement of any proteins which are differentially represented in the cytosol, mitochondria and nuclei, and is particularly applicable to studies of proteins that translocate between these three cellular compartments.

     

    A second version of the kit is also available, ab109719 Cell Fractionation Kit - Standard. This version is designed for fractionating cells grown in batches and is ideal for experiments requiring larger sample amounts.

     

  • Notes

    Storage: Store Buffer A, Detergent HT II, Detergent HT III and 5X SDS Sample Buffer at -20°C. Store Detergent HT I at -80°C. Store the collection plates and seal films at room temperature.

  • Tested applicationsFunctional Studiesmore details

Properties

  • Alternative names
    • MS862

Applications

Our Abpromise guarantee covers the use of ab109718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Cell Fractionation Kit - High Throughput (HT) images

  • Measurement and distribution of proteins using Western blot, which are differentially represented in the cytosol, mitochondria and nuclei after treatment with varying concentrations of staurosporine.

Protocols

References for Cell Fractionation Kit - High Throughput (HT) (ab109718)

This product has been referenced in:

See 1 Publication for this product

Product Wall

We have not tested localization of plasma membrane proteins or cytoplasmic organelles (besides mitochondria) with ab109718. However, we have tested distribution of these compartments using ab139409, a kit which works on the same principal as ab109718. ...

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ab109718 is based on sequential and selective extraction of cytosolic, mitochondrial and nuclear proteins with detergents that allow sequential release of cytosolic and mitochondrial proteins to the extracellular buffer. In the first step, the plasma m...

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Thank you for contacting us and you interest in our products.
Unfortunately I can now confirm that the Cell Fractionation Kit - HT (ab109718) is not suitable for use with tissue samples. However, we do have some other kits which you may want to co...

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Thank you for your enquiry and your interest in our products.

I can confirm that both kits can be used for mammalian cells (including mouse samples).

If you need any further assistance in the future, please do not hesitate to contac...

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Thank you fro your enquiry.

I am sorry to confirm we would not recommend to use ab109718 Cell Fractionation Kit - HTor ab109719 Cell Fractionation Kit Standardfor isolation of mitochondria from tissue. They are designed for cell culture sing...

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Thank you for calling Abcam earlier. For the Mitochondria Isolation Kit for Cultured Cells (ab110170), if you want to isolate the cytosolic fraction, you could keep the supernatant after 'SPIN 2' (see attached protocol) and that would give you a crude ...

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This is quite a common question. The fractionation kit is good for easily and rapidly separating cytosol, Mitochondria and nuclei.  This is a great method for making fractions to follow movement of proteins between these compartments such, but not limi...

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I now have some further information relating to the Cell Fractionation Kit - HT (ab109718) we discussed over the phone. The fractionation kit is good for easily and rapidly separating cytosol, Mitochondria and nuclei.  This is a great method for ma...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarant...

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Thanks so much for contacting us and for your interest in ab109718. We only have the full size kits for sale and don't have sample sizes, however we fully guarantee this kit to work as stated on the datasheet for up to 6 months after purchase. If it di...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"