Abcam's Cell Viability assay kits are a set of tools for monitoring cell viability and cellular functions. The proprietary far red fluorescent dye used in the kit is a hydrophobic compound that can easily permeate intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly red fluorescent hydrophilic product that is well-retained in the cell cytoplasm. When well excited with the He-Ne Laser at 633 nm, the red fluorophore emits intense red fluorescence at ~660 nm.
ab112123 is an effective tool of labeling cells for the investigations of cellular functions. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. ab112123 is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. It comes with reagents sufficient to perform 200 assays in a 96-well plate or 800 assays in a 384-well plate. The kit provides all the essential components with an optimized protocol for microplate reader analysis of cell viability.
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ab112123 should be stored desiccated.
|Components||2 x 96 tests|
|Assay Buffer||2 x 10ml|
|Cell NIR fluorescent dye||2 vials|
|DMSO||1 x 100µl|
Our Abpromise guarantee covers the use of ab112123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab112123 has not yet been referenced specifically in any publications.