Overview

  • Product nameAnti-Cellubrevin antibody
    See all Cellubrevin primary antibodies
  • Description
    Rabbit polyclonal to Cellubrevin
  • SpecificityReacts specifically with 10kDa protein of rat CP12 cell extract.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Does not react with: Dog, Human
  • Immunogen

    15 residue N-terminal synthetic peptide (Rat).

Properties

Applications

Our Abpromise guarantee covers the use of ab2102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/1000.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionSNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
  • Sequence similaritiesBelongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationMembrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEB antibody
    • Cellubrevin antibody
    • Synaptobrevin 3 antibody
    • Synaptobrevin-3 antibody
    • VAMP 3 antibody
    • VAMP-3 antibody
    • VAMP3 antibody
    • VAMP3_HUMAN antibody
    • Vesicle associated membrane protein 3 antibody
    • Vesicle-associated membrane protein 3 antibody
    see all

Anti-Cellubrevin antibody images

  • Anti-Cellubrevin antibody (ab2102) at 1/1000 dilution + Rat RBL-2H3 whole cell lysates at 10 µg

    Secondary
    Goat anti-rabbit polyclonal HRP conjugate at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Additional bands at : 14 kDa (possible post-translational modification).

    Exposure time : 5 seconds

    Image is courtesy of an anonymous AbReview.

    Blot was blocked with 5% milk for 1 hour at 25°C.

    See Abreview

  • This picture was kindly supplied as part of the review submitted by Jennifer Chua . The image shows staining in the perinuclear region and in the peripheral endosomal structures of RAW 264.7 cells stained with ab2102 (at a dilution of 1/50 with a secondary antibody conjugated with Alexa 568).

References for Anti-Cellubrevin antibody (ab2102)

This product has been referenced in:
  • Imai A  et al. Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane. Arch Oral Biol 48:597-604 (2003). Read more (PubMed: 12828989) »
  • Yamamoto M  et al. Reversible suppression of glutamatergic neurotransmission of cerebellar granule cells in vivo by genetically manipulated expression of tetanus neurotoxin light chain. J Neurosci 23:6759-67 (2003). Read more (PubMed: 12890769) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (RBL-2H3 cell)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification RBL-2H3 cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Sep 08 2015

Application Western blot
Sample Mouse Cell lysate - whole cell (J774 macrophage)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification J774 macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jun 15 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 0.5% · Temperature: 25°C
Sample Mouse Cell (J774 macrophage)
Specification J774 macrophage
Permeabilization Yes - 0.05% saponin
Fixative Paraformaldehyde
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Submitted May 28 2014

Dear Carmen, I am sorry that you received this shortage. I am sending you a free of charge replacement for this item. I apologize for the inconvenience. Best regards, Bill Bill Campbell, Ph.D. Technical Specialist Abcam, Inc. One Kenda...

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Regarding ab2102 and ab2103, unfortunately the peptide sequences are not available. If you have any additional questions, please let us know.

Thank you for your enquiry. Cellubrevin is an alternative name for VAMP3, and so these 3 antibodies target the same protein. Ab2103 has been tested for cross-reactivity with Human and Mouse, and does not cross-react with Rat or Dog. Ab2102 cro...

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This antibody works in immunofluorescence, the suggested working dilution is 1/50. It reacts with rat cellubrevin; rat PC12 cell extract is positive in Western blotting. It should certainly work for IF with CHO cells transfected with rat cellubrevin. (...

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The antibody has not been tested for cross-reactivity with mouse. However, the antibody was raised against a synthetic peptide from the rat N-terminal region, and is thought likely to recognise mouse.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"