Overview

  • Product name
  • Description
    Rabbit polyclonal to Cellubrevin
  • Tested applications
    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Cellubrevin.

    (Peptide available as ab43579.)

  • Positive control
    • ab43080 gave a positive signal in the following Tissue Lysates: Mouse Brain (Data not shown), Mouse Testis, Rat Brain and Rat Testis. It also gave a positive result in PC12 cell line. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab43080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration. PubMed: 22936810
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
  • Sequence similarities
    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEB antibody
    • Cellubrevin antibody
    • Synaptobrevin 3 antibody
    • Synaptobrevin-3 antibody
    • VAMP 3 antibody
    • VAMP-3 antibody
    • VAMP3 antibody
    • VAMP3_HUMAN antibody
    • Vesicle associated membrane protein 3 antibody
    • Vesicle-associated membrane protein 3 antibody
    see all

Images

  • All lanes : Anti-Cellubrevin antibody (ab43080) at 1 µg/ml

    Lane 1 : Testis (Mouse) Mouse Tissue Lysate
    Lane 2 : Brain (Rat) Rat Tissue Lysate
    Lane 3 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 13 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab43080 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab43080, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of Cellubrevin staining in Mouse normal testes formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab43080, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Ligeon LA  et al. Role of VAMP3 and VAMP7 in the commitment of Yersinia pseudotuberculosis to LC3-associated pathways involving single- or double-membrane vacuoles. Autophagy 10:1588-602 (2014). Read more (PubMed: 25046114) »
  • Garcia EA  et al. Characterization of SNARE proteins in human pituitary adenomas: targeted secretion inhibitors as a new strategy for the treatment of acromegaly? J Clin Endocrinol Metab 98:E1918-26 (2013). Read more (PubMed: 24152687) »

See all 4 Publications for this product

Customer reviews and Q&As


I have run a BLAST for the immunogen of VAMP3 and according to this it is specific to this protein. Experimentally I cannot provide further details as we have not tested it in house.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (Oligodendrocytes)
Total protein in input
50 µg
Specification
Oligodendrocytes
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted Feb 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Fibroblast)
Specification
Fibroblast
Fixative
Formaldehyde
Permeabilization
Yes - 0.03% Trition
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Astrocytes)
Loading amount
30 µg
Specification
Astrocytes
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 25 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (brain sections)
Specification
brain sections
Username

Dr. Sophie Pezet

Verified customer

Submitted Sep 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up