Anti-Cellular Apoptosis Susceptibility antibody (ab96755)

Overview

  • Product nameAnti-Cellular Apoptosis Susceptibility antibody
    See all Cellular Apoptosis Susceptibility primary antibodies
  • Description
    Rabbit polyclonal to Cellular Apoptosis Susceptibility
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 222-439 of Human Cellular Apoptosis Susceptibility (NP_001307)

  • Positive control
    • 293T, A431, H1299, HeLa whole cell lysates. HepG2, Molt-4 and Raji Lysates. Cal27 Xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab96755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 110 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH 8.0)
ICC/IF 1/100 - 1/200.

Target

  • FunctionExport receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • Tissue specificityHighly expressed in proliferating cells.
  • Sequence similaritiesBelongs to the XPO2/CSE1 family.
    Contains 1 importin N-terminal domain.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAS antibody
    • Cellular apoptosis susceptibility protein antibody
    • Chromosome segregation 1 (yeast homolog) like antibody
    • Chromosome segregation 1 Like antibody
    • Chromosome segregation 1 like protein antibody
    • Chromosome segregation 1-like protein antibody
    • Chromosome segregation gene CSE1 antibody
    • CSE 1 antibody
    • CSE 1 chromosome segregation 1 like antibody
    • CSE 1 chromosome segregation 1 like protein antibody
    • CSE 1L antibody
    • CSE1 antibody
    • CSE1 chromosome segregation 1 like (yeast) antibody
    • CSE1 chromosome segregation 1 like antibody
    • CSE1 chromosome segregation 1 like protein antibody
    • CSE1L antibody
    • Exp 2 antibody
    • Exp2 antibody
    • Exportin 2 antibody
    • Exportin-2 antibody
    • Exportin2 antibody
    • Importin alpha re exporter antibody
    • Importin-alpha re-exporter antibody
    • MGC117283 antibody
    • MGC130036 antibody
    • MGC130037 antibody
    • XPO 2 antibody
    • XPO2 antibody
    • XPO2_HUMAN antibody
    see all

Anti-Cellular Apoptosis Susceptibility antibody images

  • All lanes : Anti-Cellular Apoptosis Susceptibility antibody (ab96755) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 110 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96755 (1) and merged with DNA probe (2) at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using ab96755 at 1/500 dilution.
  • All lanes : Anti-Cellular Apoptosis Susceptibility antibody (ab96755) at 1/2500 dilution

    Lane 1 : Hela extracts 25 ug
    Lane 2 : RPE-1 extracts 25 ug
    Lane 3 : Hela extracts 50 ug
    Lane 4 : RPE-1 extracts 50 ug

    Secondary
    Donkey LICOR anti-rabbit IRDye680 at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa (possible cross reactivity),70 kDa (possible cross reactivity).

    Exposure time : 3 minutes

    See Abreview

References for Anti-Cellular Apoptosis Susceptibility antibody (ab96755)

ab96755 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa and RPE-1)
Loading amount 25 µg
Specification HeLa and RPE-1
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris (MES Buffer))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"