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Abcam's Cellular Membrane Potential Assay Kit (Fluorometric - Red) (ab176765) is a homogeneous assay with fast read time. It uses a proprietary long wavelength membrane potential indicator to detect the membrane potential change that is caused by the opening and closing of the ion channels. The red fluorescence of the membrane potential indicator used in the kit has enhanced fluorescence upon entering cells and minimizes the interferences resulted from the screening compounds and/or cellular autofluorescence.
Membrane potential is the difference in voltage between the interior and exterior of a cell. The membrane potential allows a cell to function as a battery, providing power to operate a variety of "molecular devices" embedded in the membrane. In electrically excitable cells such as neurons, membrane potential is used for transmitting signals between different parts of a cell. Opening or closing of ion channels at one point in the membrane produces a local change in the membrane potential, which causes electric current to flow rapidly to other points in the membrane. Ion channels have been identified as important drug discovery targets.
|Components||1 x 96 tests||10 x 96 tests||100 x 96 tests|
|10X MP Sensor||1 x 1ml||1 x 10ml||10 x 10ml|
|HHBS||1 x 9ml||1 x 100ml||0 x 0ml|
Our Abpromise guarantee covers the use of ab176765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
HEK cells transiently transfected with P2X receptor were seeded overnight at 40,000 cells/100 µL/well in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL of the MP sensor in a 5% CO2, 37 °C incubator for 60 minutes. ATP (50 µL/well) was added to achieve the final indicated concentrations. The fluorescence signal was measured with bottom read mode at Ex/Em = 620/650 nm (cutoff at 630 nm).
ab176765 has not yet been referenced specifically in any publications.