• Product name
  • Description
    Rabbit polyclonal to CELSR2
  • Tested applications
    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Dog, Macaque monkey
  • Immunogen

    Synthetic peptide within Human CELSR2 aa 2600-2700 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    (Peptide available as ab114002)

  • Positive control
    • This antibody gave a positive signal in Human Brain Tissue Lysate.



Our Abpromise guarantee covers the use of ab90817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 317 kDa (predicted molecular weight: 317 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    Receptor that may have an important role in cell/cell signaling during nervous system formation.
  • Tissue specificity
    Highest expression in brain and testis.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 9 cadherin domains.
    Contains 7 EGF-like domains.
    Contains 1 GPS domain.
    Contains 1 laminin EGF-like domain.
    Contains 2 laminin G-like domains.
  • Post-translational
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cadherin EGF LAG seven pass G type receptor 2 flamingo Drosophila homolog antibody
    • Cadherin EGF LAG seven-pass G-type receptor 2 antibody
    • Cadherin family member 10 antibody
    • CDHF10 antibody
    • CELR2_HUMAN antibody
    • Celsr2 antibody
    • EGF like domain multiple 2 antibody
    • EGF-like protein 2 antibody
    • EGFL2 antibody
    • epidermal growth factor like 2 antibody
    • Epidermal growth factor-like protein 2 antibody
    • Flamingo homolog 3 antibody
    • Flamingo1 antibody
    • KIAA0279 antibody
    • MEGF3 antibody
    • Multiple EGF-like domains protein 3 antibody
    • Multiple epidermal growth factor-like domains 3 antibody
    • Multiple epidermal growth factor-like domains protein 3 antibody
    see all

Anti-CELSR2 antibody images

  • CELSR2 was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to CELSR2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab90817.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 317kDa; CELSR2

  • Anti-CELSR2 antibody (ab90817) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 317 kDa
    Observed band size : 317 kDa

    Exposure time : 16 minutesAbcam recommends loading unheated lysates and using 20% Ethanol and 0.04% SDS in the transfer buffer to obtain a positive signal for this antibody. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above
  • ICC/IF image of ab90817 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90817 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CELSR2 antibody (ab90817)

ab90817 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Fresh Frozen Sections of Mouse In Vivo Oral Epithe)
Yes - 0.05% Triton-X 100
Fresh Frozen Sections of Mouse In Vivo Oral Epithe
Blocking step
Gelatin (Sigma G7765) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Dec 22 2015

Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Mouse Tissue sections (Adult Female reproductive - Ovary/Oviduct/Uterus)
Adult Female reproductive - Ovary/Oviduct/Uterus
Yes - PBST (PBS/0.1% v/v TritonX100 (sigma))

Ms. Julia Watson

Verified customer

Submitted Nov 24 2014


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