Overview

  • Product name
    Anti-Cenexin1/ODF2 antibody
    See all Cenexin1/ODF2 primary antibodies
  • Description
    Goat polyclonal to Cenexin1/ODF2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Dog, Human, Pig
  • Immunogen

    Synthetic peptide:

    C-REKHQASQKENKQ

    , corresponding to internal sequence amino acids 649-661 of Human Cenexin1/ODF2 (NP_002531.3; NP_702915.1).

  • Positive control
    • C57/B6 mouse CTL lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab121023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 95 kDa.

Target

  • Function
    Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
  • Tissue specificity
    Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails.
  • Sequence similarities
    Belongs to the ODF2 family.
  • Post-translational
    modifications
    Tyrosine phosphorylated.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles.
  • Information by UniProt
  • Database links
  • Alternative names
    • 84 kDa outer dense fiber protein antibody
    • Cancer/testis antigen 134 antibody
    • Cenexin 1 antibody
    • Cenexin antibody
    • Cenexin1 variant 1 antibody
    • CT134 antibody
    • FLJ44866 antibody
    • KKT 4 antibody
    • KKT4 antibody
    • MGC111096 antibody
    • MGC9034 antibody
    • ODF 2 antibody
    • ODF 84 antibody
    • odf2 antibody
    • ODF2/1 antibody
    • ODF2/2 antibody
    • ODF84 antibody
    • ODFP2_HUMAN antibody
    • OTTHUMP00000022274 antibody
    • OTTHUMP00000022275 antibody
    • Outer dense fiber of sperm tail 2 antibody
    • Outer dense fiber of sperm tails 2 antibody
    • Outer dense fiber of sperm tails protein 2 antibody
    • Outer dense fiber of sperm tails, 84 kD antibody
    • Outer dense fiber protein 2 antibody
    • Sperm outer dense fiber major protein 2 antibody
    • Sperm tail structural protein antibody
    • Testis specific autoantigen antibody
    see all

Images

References

This product has been referenced in:
  • Nixon B  et al. The Australian saltwater crocodile (Crocodylus porosus) provides evidence that the capacitation of spermatozoa may extend beyond the mammalian lineage. Proc Biol Sci 283:N/A (2016). Read more (PubMed: 27147099) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (RPE1 (retina cell line))
Permeabilization
Yes - Triton-X-100 0,1%
Specification
RPE1 (retina cell line)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37¯°C
Fixative
Methanol
Username

Miss. Karine Monier

Verified customer

Submitted Sep 13 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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