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Products:Epigenetics and Nuclear Signaling >> Chromosome Structure >> Centromeres
Anti-Cenexin1/ODF2 antibody (ab43840)
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Proteins and Peptides
ab43839
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab43840? Please let us know so that we can cite the reference in this datasheet
ab43840 has been referenced in 1 publications.
- Wilsch-Bräuninger M et al. Basolateral rather than apical primary cilia on neuroepithelial cells committed to delamination. Development 139:95-105 (2012). ICC/IF; Mouse. PubMed: 22096071
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - Cenexin1/ODF2 antibody (ab43840)
ab43840 staining Cenexin1 in the mother centriole of a dividing HeLa cell by ICC/IF. DNA is stained blue, Aurora A is stained red, and Cenexin1 is stained green. Cells were fixed in formaldehyde, permeabilized with 0.1% Triton in PBS, and blocked with 2% BSA for 1 hour at 21ºC. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 21ºC. A FITC-conjugated anti-rabbit IgG H+L antibody was used as the secondary.
Image courtesy of an anonymous abreview
Western blot - Cenexin1/ODF2 antibody (ab43840)
All lanes : Anti-Cenexin1/ODF2 antibody (ab43840) at 1/500 dilution Image courtesy of an anonymous abreview.
Lane 1 : 25 ug HeLa cell lysate
Lane 2 : 12.5 ug HeLa cell lysate
Lane 3 : 25 ug HeLa cell lysate with
Lane 4 : 12.5 ug HeLa cell lysate with
Secondary
Anti-Rabbit IgG HRP-conjugated at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 93 kDa
Observed band size : ~75 kDa (why is the actual band size different from the predicted?)
Exposure time : 4 minutes
Detection of Cenexin 1 by ab43840 in Western blot of HeLa cell lysates. Please see anonymous abreview for further details.
Cenexin1/ODF2 antibody for ICC/IF in Human (43840)
Cenexin1/ODF2 antibody for WB in Human (43840)
Cenexin1/ODF2 antibody for Immunocytochemistry/ Immunofluorescence in Human (43840)
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