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Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human Cenexin1/ODF2.
(Peptide available as ab43839.)
Our Abpromise guarantee covers the use of ab43840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22096071|
Immunocytochemistry/ Immunofluorescence analysis of human HCT116 cells labeling Cenexin1/ODF2 with ab43840 at 1/400 dilution. Cells were fixed in formaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab43840 at 1/400 was carried out for 1 hour at 22°C in PBS buffer. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution. DAPI was used to counterstain.
Image courtesy of an anonymous abreview.Detection of Cenexin 1 by ab43840 in Western blot of HeLa cell lysates. Please see anonymous abreview for further details.