• Product name
  • Description
    Rabbit polyclonal to CENPA
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: MGPRRRSRKP EAPRRRSPSP TPTPGPSRRG PSLGASSHQH SRRRQGWLKE, corresponding to a region within N terminal amino acids 1-50 of Human CENPA

  • Positive control
    • Jurkat cell lysate. Human skin.



Our Abpromise guarantee covers the use of ab65678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
  • Sequence similarities
    Belongs to the histone H3 family.
  • Domain
    The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.
  • Post-translational
    Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
    Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.
  • Information by UniProt
  • Database links
  • Alternative names
    • CENP A antibody
    • CENP-A antibody
    • cenpa antibody
    • CENPA_HUMAN antibody
    • Centromere autoantigen A antibody
    • Centromere protein A 17kDa antibody
    • Centromere protein A antibody
    • Histone H3 like centromeric protein A antibody
    • Histone H3-like centromeric protein A antibody
    see all

Anti-CENPA antibody images

  • Anti-CENPA antibody (ab65678) at 1.25 µg/ml + Jurkat cell lysate

    Predicted band size : 16 kDa
  • Immunohistochemical analysis of paraffin-embedded human skin tissue stained with ab65678 at 4µg/ml. Positively stained squamous epithelial cells are indicated with arrows.

References for Anti-CENPA antibody (ab65678)

ab65678 has not yet been referenced specifically in any publications.

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