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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CENPA aa 100 to the C-terminus (C terminal).
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
Our Abpromise guarantee covers the use of ab45694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/20000. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).|
See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).
Immunohistochemical analysis of paraffin embedded human colon tissue section labelling CENPA with purified ab45694 at dilution of 1/250. The secondary antibody used was HRP-conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
IHC image of ab45694 staining CENPA in Human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab45694, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemical analysis of formalin fixed, paraffin embedded colonic adenocarcinoma tissue section labelling CENPA with unpurified ab45694.
Immunohistochemical analysis of formalin fixed, paraffin embedded Lymphoma tissue section labelling CENPA with unpurified ab45694.
Immunohistochemical analysis of formalin fixed, paraffin embedded prostatic carcinoma tissue section labelling CENPA with unpurified ab45694.
Immunohistochemical analysis of formalin fixed, paraffin embedded lung carcinoma tissue section labelling CENPA with unpurified ab45694.
ab45694 has not yet been referenced specifically in any publications.