Overview

  • Product name
  • Description
    Mouse polyclonal to CENPB
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human protein corresponding to amino acids 1-599 of Human CENPB (AAH53847.1).

  • Positive control
    • A431 cell lysate, CENPB transfected 293T cell line lysate, HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.
  • Sequence similarities
    Contains 1 HTH CENPB-type DNA-binding domain.
    Contains 1 HTH psq-type DNA-binding domain.
  • Cellular localization
    Nucleus. Chromosome > centromere.
  • Information by UniProt
  • Database links
  • Alternative names
    • CENP B antibody
    • CENP-B antibody
    • CENPB antibody
    • CENPB_HUMAN antibody
    • centromere autoantigen B antibody
    • Centromere protein B antibody
    • Centromere protein B, 80kDa antibody
    • Major centromere autoantigen B antibody
    see all

Images

  • Anti-CENPB antibody (ab167361) at 1 µg/ml + A431 cell lysate at 50 µg

    Secondary
    Goat anti-Mouse IgG HRP at 1/2500 dilution

    Predicted band size: 65 kDa

  • All lanes : Anti-CENPB antibody (ab167361) at 1 µg/ml

    Lane 1 : CENPB transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG HRP at 1/2500 dilution

    Predicted band size: 65 kDa

  • Immunofluorescence analysis of HeLa cells labeling CENPB using ab167361 at 10 µg/ml.

References

ab167361 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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