Overview

  • Product name
  • Description
    Rabbit polyclonal to CENPB
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residues 540 and the C-terminus (residue 599) of human CENPB using the numbering given in entry SwissProt entry P07199

  • Positive control
    • HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified using an epitope specific to CENP-B immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    ICC/IF: 1/100 - 1/500.
    Permeabilization with Triton-X 100 is recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.
    • Sequence similarities
      Contains 1 HTH CENPB-type DNA-binding domain.
      Contains 1 HTH psq-type DNA-binding domain.
    • Cellular localization
      Nucleus. Chromosome > centromere.
    • Information by UniProt
    • Database links
    • Alternative names
      • CENP B antibody
      • CENP-B antibody
      • CENPB antibody
      • CENPB_HUMAN antibody
      • centromere autoantigen B antibody
      • Centromere protein B antibody
      • Centromere protein B, 80kDa antibody
      • Major centromere autoantigen B antibody
      see all

    Anti-CENPB antibody images

    • ICC analysis of NBF-fixed asynchronous HeLa cells using ab84489 at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC-conjugated antibody used at a dilution of 1/100.
    • ICC analysis of NBF-fixed asynchronous HeLa cells using ab84489 at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC-conjugated antibody used at a dilution of 1/100.

    References for Anti-CENPB antibody (ab84489)

    ab84489 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab84489.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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