Overview

  • Product nameAnti-CENPC antibody [2159C5a]
    See all CENPC primary antibodies
  • Description
    Mouse monoclonal [2159C5a] to CENPC
  • Tested applicationsSuitable for: WB, Dot blot, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab50974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 107 kDa.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/250.

Use at a dilution of 1/250 (for overnight incubation).

Target

  • FunctionComponent of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions.
  • Sequence similaritiesBelongs to the CENPC family.
  • DomainThe MIF2 homology domain II targets centromeres and binds the alpha satellite DNA in vivo. The MIF2 homology domain III can induce CENPC dimerization/oligomerization.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres.
  • Information by UniProt
  • Database links
  • Alternative names
    • CENP-C 1 antibody
    • CENP-C antibody
    • CENPC_HUMAN antibody
    • CENPC1 antibody
    • Centromere autoantigen C antibody
    • Centromere autoantigen C1 antibody
    • Centromere protein C 1 antibody
    • Centromere protein C antibody
    • hcp-4 antibody
    • ICEN7 antibody
    • Interphase centromere complex protein 7 antibody
    • MIF2 antibody
    see all

Anti-CENPC antibody [2159C5a] images

  • ab36406 staining human lymphoblastoid cells by ICC/IF.  Cells were PFA fixed and blocked with 1xPBS+1%BSA+0.5%Triton X-100 for 30 minutes at 25°C, prior to incubating with ab36406, diluted 1/250 for 24 hours at 4°C.  An Alexa Fluor® 488 conjugated donkey anti-mouse antibody was used as the secondary.

    See Abreview

References for Anti-CENPC antibody [2159C5a] (ab50974)

This product has been referenced in:
  • Aldrup-MacDonald ME  et al. Genomic variation within alpha satellite DNA influences centromere location on human chromosomes with metastable epialleles. Genome Res 26:1301-1311 (2016). Read more (PubMed: 27510565) »
  • Kim S & Yu H Multiple assembly mechanisms anchor the KMN spindle checkpoint platform at human mitotic kinetochores. J Cell Biol 208:181-96 (2015). Read more (PubMed: 25601404) »

See all 6 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (LLCPK1 pig kidney epithelial cells)
Permeabilization No
Specification LLCPK1 pig kidney epithelial cells
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 17 2016

Thank you for contacting Abcam regarding ab50974.

The immunogen sequence is as follows: XXX Please contact us for additional details.

I hope this information is helpful. Please do not hesitate to contact me if you have any additional...

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I have forwarded the query to our reference team who are going to look into this matter and make any changes to the datasheet that are necessary.

Once again, I apologize for the problems you were having with this antibody.

Please le...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human lymphoblastoid)
Specification human lymphoblastoid
Fixative Paraformaldehyde
Blocking step 1XPBS+1%BSA+0.5%TritonX-100 as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Beth Sullivan

Verified customer

Submitted May 21 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"