Anti-CENPF antibody (ab84697)
Key features and details
- Rabbit polyclonal to CENPF
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CENPF antibody
See all CENPF primary antibodies -
Description
Rabbit polyclonal to CENPF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide:
QAEIQIKEES KTAVEMLQN Q LKELNEAV AA LCGDQEI MKA TEQSLD PPIE EEHQL RNSIE
, corresponding to amino acids 2350-2400 of Human CENPF -
Positive control
- IHC: human ewing sarcoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab84697 was affinity purified using an epitope specific to CENPF immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. -
Involvement in disease
Stromme syndrome -
Sequence similarities
Belongs to the centromere protein F family. -
Developmental stage
Gradually accumulates during the cell cycle, reaching peak levels in G2 and M phase, and is rapidly degraded upon completion of mitosis. -
Post-translational
modificationsHyperphosphorylated during mitosis. -
Cellular localization
Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body. - Information by UniProt
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Database links
- Entrez Gene: 1063 Human
- Omim: 600236 Human
- SwissProt: P49454 Human
- Unigene: 497741 Human
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Alternative names
- AH antigen antibody
- Cell cycle dependent 350K nuclear protein antibody
- CENF antibody
see all
Images
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ab84697, at a dilution of 1/100, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
Detection: Red fluorescent goat anti-rabbit IgG highly cross adsorbed antibody used at a dilution of 1/100. -
ab84697, at a dilution of 1/500, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
Detection: DAB staining -
ab84697, at a dilution of 1/500, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
Detection: DAB staining -
ab84697, at a dilution of 1/100, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
Detection: DAB staining
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab84697 has been referenced in 1 publication.
- Han Y et al. CENPF promotes papillary thyroid cancer progression by mediating cell proliferation and apoptosis. Exp Ther Med 21:401 (2021). PubMed: 33680123