Overview

  • Product nameAnti-CENPF antibody
    See all CENPF primary antibodies
  • Description
    Rabbit polyclonal to CENPF
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide:

    QAEIQIKEES KTAVEMLQN Q LKELNEAV AA LCGDQEI MKA TEQSLD PPIE EEHQL RNSIE

    , corresponding to amino acids 2350-2400 of Human CENPF

  • Positive control
    • Human controls: Breast Carcinoma, Colon Carcinoma, Ewing Sarcoma, Stomach Adenocarcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab84697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionRequired for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.
  • Involvement in diseaseStromme syndrome
  • Sequence similaritiesBelongs to the centromere protein F family.
  • Developmental stageGradually accumulates during the cell cycle, reaching peak levels in G2 and M phase, and is rapidly degraded upon completion of mitosis.
  • Post-translational
    modifications
    Hyperphosphorylated during mitosis.
  • Cellular localizationCytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • AH antigen antibody
    • Cell cycle dependent 350K nuclear protein antibody
    • CENF antibody
    • CENP F antibody
    • CENP-F antibody
    • CENPF antibody
    • CENPF kinetochore protein antibody
    • CENPF_HUMAN antibody
    • Centromere protein F 350/400ka antibody
    • Centromere protein F antibody
    • Hcp 1 antibody
    • Hcp1 antibody
    • Kinetochore protein CENP F antibody
    • Kinetochore protein CENPF antibody
    • Mitosin antibody
    • PRO1779 antibody
    see all

Anti-CENPF antibody images

  • ab84697 staining CENPF in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG H&L polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

    See Abreview

  • ab84697, at a dilution of 1/100, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
    Detection: Red fluorescent goat anti-rabbit IgG highly cross adsorbed antibody used at a dilution of 1/100.
  • ab84697, at a dilution of 1/500, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
    Detection: DAB staining
  • ab84697, at a dilution of 1/500, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
    Detection: DAB staining
  • ab84697, at a dilution of 1/100, staining CENPF in formalin fixed, paraffin embedded human ewing sarcoma by Immunohistochemistry.
    Detection: DAB staining

References for Anti-CENPF antibody (ab84697)

ab84697 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Jul 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"