Overview

  • Product nameAnti-CENPF antibody
    See all CENPF primary antibodies
  • Description
    Rabbit polyclonal to CENPF
  • Tested applicationsSuitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1800-1850 of Human CENPF

  • Positive control
    • HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab93236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notesICC/IF: 1/100 - 1/500.
    Acetone fixation should be used. Formaldehyde or methanol fixation is not recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.
    • Involvement in diseaseStromme syndrome
    • Sequence similaritiesBelongs to the centromere protein F family.
    • Developmental stageGradually accumulates during the cell cycle, reaching peak levels in G2 and M phase, and is rapidly degraded upon completion of mitosis.
    • Post-translational
      modifications
      Hyperphosphorylated during mitosis.
    • Cellular localizationCytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.
    • Information by UniProt
    • Database links
    • Alternative names
      • AH antigen antibody
      • Cell cycle dependent 350K nuclear protein antibody
      • CENF antibody
      • CENP F antibody
      • CENP-F antibody
      • CENPF antibody
      • CENPF kinetochore protein antibody
      • CENPF_HUMAN antibody
      • Centromere protein F 350/400ka antibody
      • Centromere protein F antibody
      • Hcp 1 antibody
      • Hcp1 antibody
      • Kinetochore protein CENP F antibody
      • Kinetochore protein CENPF antibody
      • Mitosin antibody
      • PRO1779 antibody
      see all

    Anti-CENPF antibody images

    • Detection of Human CENPF in Acetone fixed asynchronous HeLa cells by Immunocytochemistry using ab93236 at a dilution of 1/250. Detection: FITC conjugated anti-rabbit IgG used at a dilution of 1/100.
    • Detection of Human CENPF in Acetone fixed asynchronous HeLa cells by Immunocytochemistry using ab93236 at a dilution of 1/250. Detection: FITC conjugated anti-rabbit IgG used at a dilution of 1/100.

    References for Anti-CENPF antibody (ab93236)

    ab93236 has not yet been referenced specifically in any publications.

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