Overview

Description

  • NatureSynthetic
  • Amino Acid Sequence
    • SequenceGLESKGSENCKVQ

Specifications

Our Abpromise guarantee covers the use of ab45477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Info

  • Alternative names
    • AH antigen
    • Cell cycle dependent 350K nuclear protein
    • CENF
    • CENP F
    • CENP-F
    • CENPF
    • CENPF kinetochore protein
    • CENPF_HUMAN
    • Centromere protein F
    • Centromere protein F 350/400ka
    • Hcp 1
    • Hcp1
    • Kinetochore protein CENP F
    • Kinetochore protein CENPF
    • Mitosin
    • PRO1779
    see all
  • FunctionRequired for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.
  • Involvement in diseaseStromme syndrome
  • Sequence similaritiesBelongs to the centromere protein F family.
  • Developmental stageGradually accumulates during the cell cycle, reaching peak levels in G2 and M phase, and is rapidly degraded upon completion of mitosis.
  • Post-translational
    modifications
    Hyperphosphorylated during mitosis.
  • Cellular localizationCytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.
  • Information by UniProt

References for CENPF peptide (ab45477)

ab45477 has not yet been referenced specifically in any publications.

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