Anti-CENPH antibody (ab88593)
Key features and details
- Mouse polyclonal to CENPH
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CENPH antibody
See all CENPH primary antibodies -
Description
Mouse polyclonal to CENPH -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein within Human CENPH aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- HeLa cell lysate. Raw 264.7 cell lysate. NIH/3T3 cell lysate. Lysate from transfected 293T cells. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. -
Sequence similarities
Belongs to the centromere protein H family. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase. - Information by UniProt
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Database links
- Entrez Gene: 64946 Human
- Entrez Gene: 26886 Mouse
- Omim: 605607 Human
- SwissProt: Q9H3R5 Human
- SwissProt: Q9QYM8 Mouse
- Unigene: 631967 Human
- Unigene: 273502 Mouse
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Alternative names
- CENP H antibody
- CENP-H antibody
- CENPH antibody
see all
Images
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Anti-CENPH antibody (ab88593) at 5 µg/ml + HeLa cell lysate at 25 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Anti-CENPH antibody (ab88593) at 5 µg/ml + Raw 264.7 cell lysate at 25 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Anti-CENPH antibody (ab88593) at 5 µg/ml + NIH/3T3 cell lysate at 25 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Additional bands at: ~19 kDa, ~60 kDa, ~70 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-CENPH antibody (ab88593) at 5 µg/ml
Lane 1 : cell lysate from CENPH transfected 293T cells
Lane 2 : cell lysate from non transfected 293T cells
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa -
ab88593 at 10 µg/ml detecting CENPH in HeLa cells by immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab88593 has been referenced in 2 publications.
- Hu L et al. Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment. Nucleic Acids Res 47:468-479 (2019). PubMed: 30407575
- Zhang T et al. CenpH regulates meiotic G2/M transition by modulating the APC/CCdh1-cyclin B1 pathway in oocytes. Development 144:305-312 (2017). PubMed: 27993978