Overview

  • Product name
  • Description
    Mouse polyclonal to CENPH
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-247 of Human CENPH (NP_075060.1).

  • Positive control
    • HeLa cell lysate. Raw 264.7 cell lysate. NIH/3T3 cell lysate. Lysate from transfected 293T cells. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
  • Sequence similarities
    Belongs to the centromere protein H family.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • CENP H antibody
    • CENP-H antibody
    • CENPH antibody
    • CENPH_HUMAN antibody
    • Centromere protein H antibody
    • ICEN35 antibody
    • Interphase centromere complex protein 35 antibody
    • Kinetochore protein CENP H antibody
    • NNF1 antibody
    • NNF1, MIND kinetochore complex component, homolog antibody
    • PMF1 antibody
    see all

Images

  • Anti-CENPH antibody (ab88593) at 5 µg/ml + HeLa cell lysate at 25 µg
    Developed using the ECL technique

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • Anti-CENPH antibody (ab88593) at 5 µg/ml + Raw 264.7 cell lysate at 25 µg
    Developed using the ECL technique

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • Anti-CENPH antibody (ab88593) at 5 µg/ml + NIH/3T3 cell lysate at 25 µg
    Developed using the ECL technique

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : ~19 kDa,~60 kDa,~70 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-CENPH antibody (ab88593) at 5 µg/ml

    Lane 1 : cell lysate from CENPH transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • ab88593 at 10 µg/ml detecting CENPH in HeLa cells by immunofluorescence.

References

This product has been referenced in:
  • Zhang T  et al. CenpH regulates meiotic G2/M transition by modulating the APC/CCdh1-cyclin B1 pathway in oocytes. Development 144:305-312 (2017). Read more (PubMed: 27993978) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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