Overview

  • Product name
  • Description
    Mouse polyclonal to CENPI
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-522 of Human CENPI, Isoform 2 (AAH12462.1, UniProt ID: Q92674-2)

  • Positive control
    • Lysate from 293T cells transfected with CENPI; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab168778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Relevance
    CENPI is a constitutive protein of the knetochore that shares limited similarity with Mis6 of S. pombe. The kinetochore is a macromolecular complex located at the centromere of chromosomes and provides essential functions for chromosome segregration. CENPI is required for the localization of CENPF and the checkpoint proteins MAD1 and MAD2 to the kinetochore.
  • Cellular localization
    Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • CENP I antibody
    • centromere protein I antibody
    • Follicle simulating hormone primary response protein antibody
    • FSH primary response 1 antibody
    • FSH primary response protein 1 antibody
    • FSH primary response, rat, homolog of, 1 antibody
    • FSHP1 antibody
    • FSHPRH1 antibody
    • interphase centromere complex protein 19 antibody
    • Leucine rich primary response gene 1 antibody
    • Leucine rich primary response protein 1 antibody
    • LRPR1 antibody
    • Mis6 antibody
    see all

Images

  • All lanes : Anti-CENPI antibody (ab168778) at 1 µg/ml

    Lane 1 : CENPI-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique.

    Predicted band size: 60 kDa

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling CENPI using ab168778 at 10 µg/ml.

References

ab168778 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab168778.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up