Overview

  • Product nameAnti-CENPJ antibody
    See all CENPJ primary antibodies
  • Description
    Rabbit polyclonal to CENPJ
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human CENPJ.

    (Peptide available as ab28894.)

  • Positive control
    • This antibody gave a positive signal in Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab26052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 149 kDa (predicted molecular weight: 153 kDa).

Target

  • FunctionPlays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome.
  • Involvement in diseaseDefects in CENPJ are the cause of microcephaly primary type 6 (MCPH6) [MIM:608393]. A disorder defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.
    Defects in CENPJ are the cause of Seckel syndrome type 4 (SCKL4) [MIM:613676]. SCKL4 is a rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation.
  • Sequence similaritiesBelongs to the TCP10 family.
  • Post-translational
    modifications
    Phosphorylation at Ser-589 and Ser-595 by PLK2 is required for procentriole formation and centriole elongation. Phosphorylation by PLK2 oscillates during the cell cycle: it increases at G1/S transition and decreases during the exit from mitosis. Phosphorylation at Ser-595 is also mediated by PLK4 but is not a critical step in PLK4 function in procentriole assembly.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Localized within the center of microtubule asters. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
  • Information by UniProt
  • Database links
  • Alternative names
    • CENP-J antibody
    • CENPJ antibody
    • CENPJ_HUMAN antibody
    • Centromere protein J antibody
    • Centrosomal P4.1-associated protein antibody
    • CPAP antibody
    • LAG-3-associated protein antibody
    • LAP antibody
    • LIP1 antibody
    • LYST-interacting protein 1 antibody
    • MCPH6 antibody
    see all

Anti-CENPJ antibody images

  • Anti-CENPJ antibody (ab26052) at 1 µg/ml + Jurkat (Human) Nuclear Lysate - CD3 activated (ab14845) at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 153 kDa
    Observed band size : 149 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CENPJ antibody (ab26052)

ab26052 has not yet been referenced specifically in any publications.

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