Overview

  • Product nameAnti-CENPM antibody
    See all CENPM primary antibodies
  • Description
    Rabbit polyclonal to CENPM
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 131-180 (CDLEVEGFRA TMAQRLVRVL QICAGHVPGV SALNLLSLLR SSEGPSLEDL) of Human CENPM (NP_076958).

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab84669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceCENPM (Centromere protein M) is a component of the CENPA-NAC (nucleosome associated) complex, a complex that plays a central role in kinetochore protein assembly, mitotic cell cycle progression and chromosome segregation.
  • Cellular localizationCytoplasmic and Nuclear. Note: Nuclear in non confluent cells and cytoplasmic in confluent or dividing cells. Localizes in the kinetochore domain of centromeres.
  • Database links
  • Alternative names
    • bK250D10.2 antibody
    • C22orf18 antibody
    • CENP M antibody
    • Centromere protein M antibody
    • ICEN 39 antibody
    • ICEN39 antibody
    • Interphase centromere complex protein 39 antibody
    • MGC861 antibody
    • OTTHUMP00000028741 antibody
    • PANE 1 antibody
    • PANE1 antibody
    • Proliferation associated nuclear element 1 antibody
    • Proliferation associated nuclear element protein 1 antibody
    see all

Anti-CENPM antibody images

  • Anti-CENPM antibody (ab84669) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 20 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : approx. 35 kDa,approx. 50 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • ab84669 staining CENPM in cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-CENPM antibody (ab84669)

ab84669 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"