Overview

  • Product name
    Anti-CENPN antibody
  • Description
    Rabbit polyclonal to CENPN
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 309-339 of Human BM039.

  • Positive control
    • MCF-7 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab104296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 40 kDa.

Target

  • Function
    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • BM 039 antibody
    • BM 309 antibody
    • BM-309 antibody
    • BM039 antibody
    • C16orf60 antibody
    • CENP N antibody
    • CENP-N antibody
    • Cenpn antibody
    • CENPN_HUMAN antibody
    • Centromere protein N antibody
    • FLJ13607 antibody
    • FLJ22660 antibody
    • ICEN 32 antibody
    • ICEN32 antibody
    • Interphase centromere complex protein 32 antibody
    see all

Images

  • Anti-CENPN antibody (ab104296) at 1/50 dilution + MCF7 cell lysate at 35 µg

    Predicted band size : 40 kDa

References

ab104296 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Dec 08 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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