Overview

  • Product nameAnti-CENPT antibody
    See all CENPT primary antibodies
  • Description
    Rabbit polyclonal to CENPT
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 520-561 of Human CENPT (NP_079358.3 ).

  • Positive control
    • HeLa whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab114120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 10 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionComponent of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function.
    • Sequence similaritiesBelongs to the CENPT family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres.
    • Information by UniProt
    • Database links
    • Alternative names
      • C16orf56 antibody
      • CENP T antibody
      • CENP-T antibody
      • Cenpt antibody
      • CENPT_HUMAN antibody
      • Centromere protein T antibody
      • Chromosome 16 open reading frame 56 antibody
      • ICEN22 antibody
      • Interphase centromere complex protein 22 antibody
      see all

    Anti-CENPT antibody images

    • Detection of CENPT by Western Blot of Immunprecipitate.
      Lane 1: An antibody to CENPT at 1µg/ml staining CENPT in HeLa whole cell lysate immunoprecipitated using ab114120 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Lane 2: Control IgG
      Detection: Chemiluminescence with exposure time of 3 seconds.

    References for Anti-CENPT antibody (ab114120)

    ab114120 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab114120.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"