Overview

  • Product name
  • Description
    Rabbit polyclonal to CENPT
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 520-561 of Human CENPT (NP_079358.3 ).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab114120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function.
    • Sequence similarities
      Belongs to the CENPT family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres.
    • Information by UniProt
    • Database links
    • Alternative names
      • C16orf56 antibody
      • CENP T antibody
      • CENP-T antibody
      • Cenpt antibody
      • CENPT_HUMAN antibody
      • Centromere protein T antibody
      • Chromosome 16 open reading frame 56 antibody
      • ICEN22 antibody
      • Interphase centromere complex protein 22 antibody
      see all

    Images

    • Detection of CENPT by Western Blot of Immunprecipitate.
      Lane 1: An antibody to CENPT at 1µg/ml staining CENPT in HeLa whole cell lysate immunoprecipitated using ab114120 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Lane 2: Control IgG
      Detection: Chemiluminescence with exposure time of 3 seconds.

    References

    ab114120 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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