Overview

  • Product nameAnti-Centaurin alpha 1 antibody
    See all Centaurin alpha 1 primary antibodies
  • Description
    Goat polyclonal to Centaurin alpha 1
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide:

    CQEYAVEAHFKHKP

    , corresponding to amino acids 362-374 of Human Centaurin alpha 1.

  • Positive control
    • Human and mouse brain lysates for Western blot.

Properties

Applications

Our Abpromise guarantee covers the use of ab27476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
ELISA 1/32000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).

Target

  • RelevanceThe centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.
  • Cellular localizationCytoplasmic, Nuclear and Plasma membrane
  • Database links
  • Alternative names
    • ArfGAP with dual PH domains 1 antibody
    • CENTA 1 antibody
    • CENTA1 antibody
    • Centaurin alpha antibody
    • GCS1L antibody
    • p42IP4 antibody
    • Putative MAPK activating protein PM25 antibody
    see all

Anti-Centaurin alpha 1 antibody images

  • All lanes : Anti-Centaurin alpha 1 antibody (ab27476) at 1 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Pig brain tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 43 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • IHC image of ab27476 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27476, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Centaurin alpha 1 antibody (ab27476)

This product has been referenced in:
  • Szatmari EM  et al. Centaurin-a1-Ras-Elk-1 signaling at mitochondria mediates ß-amyloid-induced synaptic dysfunction. J Neurosci 33:5367-74 (2013). WB ; Rat . Read more (PubMed: 23516302) »
  • Moore CD  et al. The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation. J Cell Sci 120:2683-93 (2007). WB, ICC/IF, IHC-P ; Human, Rat . Read more (PubMed: 17635995) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"