Recombinant
RabMAb

Anti-Centaurin alpha 1 antibody [EPR14285] (ab183038)

Overview

  • Product name
    Anti-Centaurin alpha 1 antibody [EPR14285]
    See all Centaurin alpha 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14285] to Centaurin alpha 1
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Centaurin alpha 1 aa 150-250. The exact sequence is proprietary.
    Database link: O75689

  • Positive control
    • human fetal brain tissue lysate, A431 cell line lysate, U87 MG cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab183038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ICC/IF 1/100.
IP 1/20 - 1/40.

Target

  • Relevance
    The centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.
  • Cellular localization
    Cytoplasmic, Nuclear and Plasma membrane
  • Database links
  • Alternative names
    • ArfGAP with dual PH domains 1 antibody
    • CENTA 1 antibody
    • CENTA1 antibody
    • Centaurin alpha antibody
    • GCS1L antibody
    • p42IP4 antibody
    • Putative MAPK activating protein PM25 antibody
    see all

Images

  • Immunoprecipitation analysis of Human fetal brain lysate labeling Centaurin alpha 1 using ab183038 at 1/50 dilution. A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed U87 MG cells labeling Centaurin alpha 1 with ab183038 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 (red). The cells are also seen stained with dapi (blue).

  • Anti-Centaurin alpha 1 antibody [EPR14285] (ab183038) at 1/20000 dilution + Human fetal brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 43 kDa
  • Anti-Centaurin alpha 1 antibody [EPR14285] (ab183038) at 1/5000 dilution + A431 cell line lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size : 43 kDa

References

ab183038 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183038.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up