Overview

  • Product name
    Anti-Centaurin beta 2 antibody
    See all Centaurin beta 2 primary antibodies
  • Description
    Goat polyclonal to Centaurin beta 2
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    EKLNRFQQDSQKF

    , corresponding to C terminal amino acids 766-778 of Human Centaurin beta 2

  • Positive control
    • Human T cell line MOLT4 lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).
ELISA 1/32000.

Target

  • Function
    GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).
  • Tissue specificity
    Widely expressed. Highest level in lung.
  • Sequence similarities
    Contains 3 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 BAR domain.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAP 2 antibody
    • ACAP2 antibody
    • ACAP2_HUMAN antibody
    • ANK repeat and PH domain-containing protein 2 antibody
    • Arf GAP with coiled coil ANK repeat and PH domain containing protein 2 antibody
    • Arf GAP with coiled coil ANK repeat and PH domains 2 antibody
    • Arf-GAP with coiled-coil antibody
    • ArfGAP with coiled coil ankyrin repeat and PH domains 2 antibody
    • CENT B 2 antibody
    • Centaurin beta2 antibody
    • Centaurin-beta-2 antibody
    • CENTB 2 antibody
    • CENTB2 antibody
    • Cnt b2 antibody
    • Cnt-b2 antibody
    • Cntb2 antibody
    • KIAA0041 antibody
    • Similar to yeast ZINC FINGER PROTEIN GCS1 antibody
    see all

Anti-Centaurin beta 2 antibody images

  • Anti-Centaurin beta 2 antibody (ab26070) at 0.3 µg/ml + NIH/3T3 cell lysate at 35 µg

    Predicted band size : 88 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • All lanes : Anti-Centaurin beta 2 antibody (ab26070) at 0.3 µg/ml

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Rat lung tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 88 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Anti-Centaurin beta 2 antibody (ab26070) at 1 µg/ml + Human T cell line MOLT4 lysate (35µg protein in RIPA buffer)
    Developed using the ECL technique

    Predicted band size : 88 kDa
    Observed band size : ~90 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour.
  • ab26070 at 5ug/ml staining Centaurin beta 2 in human lung tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

References for Anti-Centaurin beta 2 antibody (ab26070)

This product has been referenced in:
  • Miyamoto Y  et al. Rab35, acting through ACAP2 switching off Arf6, negatively regulates oligodendrocyte differentiation and myelination. Mol Biol Cell 25:1532-42 (2014). WB . Read more (PubMed: 24600047) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab26070.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up