Overview

  • Product name
    Anti-Centaurin beta 2 antibody
    See all Centaurin beta 2 primary antibodies
  • Description
    Goat polyclonal to Centaurin beta 2
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    EKLNRFQQDSQKF

    , corresponding to C terminal amino acids 766-778 of Human Centaurin beta 2

  • Positive control
    • Human T cell line MOLT4 lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).
ELISA 1/32000.

Target

  • Function
    GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).
  • Tissue specificity
    Widely expressed. Highest level in lung.
  • Sequence similarities
    Contains 3 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 BAR domain.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAP 2 antibody
    • ACAP2 antibody
    • ACAP2_HUMAN antibody
    • ANK repeat and PH domain-containing protein 2 antibody
    • Arf GAP with coiled coil ANK repeat and PH domain containing protein 2 antibody
    • Arf GAP with coiled coil ANK repeat and PH domains 2 antibody
    • Arf-GAP with coiled-coil antibody
    • ArfGAP with coiled coil ankyrin repeat and PH domains 2 antibody
    • CENT B 2 antibody
    • Centaurin beta2 antibody
    • Centaurin-beta-2 antibody
    • CENTB 2 antibody
    • CENTB2 antibody
    • Cnt b2 antibody
    • Cnt-b2 antibody
    • Cntb2 antibody
    • KIAA0041 antibody
    • Similar to yeast ZINC FINGER PROTEIN GCS1 antibody
    see all

Images

  • Anti-Centaurin beta 2 antibody (ab26070) at 0.3 µg/ml + NIH/3T3 cell lysate at 35 µg

    Predicted band size : 88 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • All lanes : Anti-Centaurin beta 2 antibody (ab26070) at 0.3 µg/ml

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Rat lung tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 88 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Anti-Centaurin beta 2 antibody (ab26070) at 1 µg/ml + Human T cell line MOLT4 lysate (35µg protein in RIPA buffer)
    Developed using the ECL technique

    Predicted band size : 88 kDa
    Observed band size : ~90 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour.
  • ab26070 at 5ug/ml staining Centaurin beta 2 in human lung tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

References

This product has been referenced in:
  • Miyamoto Y  et al. Rab35, acting through ACAP2 switching off Arf6, negatively regulates oligodendrocyte differentiation and myelination. Mol Biol Cell 25:1532-42 (2014). WB . Read more (PubMed: 24600047) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab26070.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up