Anti-PIKE antibody (ab21271)
Key features and details
- Rabbit polyclonal to PIKE
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-PIKE antibody
See all PIKE primary antibodies -
Description
Rabbit polyclonal to PIKE -
Host species
Rabbit -
Specificity
Recognises PIKE: also detect both PIKE-L and PIKE-A isoforms. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Human PIKE (C terminal).
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Positive control
- Mouse brain cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified by ion exchange chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 150, 80 kDa (predicted molecular weight: 90 kDa).
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 150, 80 kDa (predicted molecular weight: 90 kDa). |
Target
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Function
GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. -
Tissue specificity
Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart. -
Sequence similarities
Belongs to the centaurin gamma-like family.
Contains 2 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 PH domain. -
Domain
G domain binds GTP and has GTPase activity.
Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
Although both PH domains of isoforms 1 and 2 bind phospholipids, they differently regulate subcellular location. PH domain of isoform 1 directs the protein to the nucleus, but PH domain of isoform 2 directs it to the cytosol. PH domain of isoform 2 is required for binding to AP-1. -
Post-translational
modificationsIsoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage. -
Cellular localization
Cytoplasm and Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 105242480 Mouse
- Entrez Gene: 216439 Mouse
- SwissProt: Q3UHD9 Mouse
- Unigene: 336761 Mouse
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Alternative names
- AGAP-2 antibody
- Agap2 antibody
- Agap2 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
see all
Images
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Lane 1 : Anti-PIKE antibody (ab21271) at 0.5 µg/ml
Lane 2 : Anti-PIKE antibody (ab21271) at 1 µg/ml
Lane 3 : Anti-PIKE antibody (ab21271) at 2 µg/ml
All lanes : Mouse brain cell lysate
Predicted band size: 90 kDa
Ab21271 at concentrations of 0.5 (lane A), 1 (lane B) and 2µ g/ml (lane C), staining PIKE-L and PIKE-A in mouse brain cell lysates by Western blot. -
ab21271 at 10µg/ml staining PIKE in mouse brain tissue by IHC
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab21271 has not yet been referenced specifically in any publications.