Anti-Centaurin gamma 1 antibody (ab21271)


  • Product nameAnti-Centaurin gamma 1 antibody
    See all Centaurin gamma 1 primary antibodies
  • Description
    Rabbit polyclonal to Centaurin gamma 1
  • SpecificityRecognises Centaurin gamma 1: also detect both PIKE-L and PIKE-A isoforms.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    This antibody was raised against a synthetic peptide corresponding to 15 amino acids near the C-terminus of human PIKE.

  • Positive control
    • Mouse brain cell lysates.



Our Abpromise guarantee covers the use of ab21271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 150, 80 kDa (predicted molecular weight: 90 kDa).


  • FunctionGTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
  • Tissue specificityIsoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart.
  • Sequence similaritiesBelongs to the centaurin gamma-like family.
    Contains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
  • DomainG domain binds GTP and has GTPase activity.
    Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
    Although both PH domains of isoforms 1 and 2 bind phospholipids, they differently regulate subcellular location. PH domain of isoform 1 directs the protein to the nucleus, but PH domain of isoform 2 directs it to the cytosol. PH domain of isoform 2 is required for binding to AP-1.
  • Post-translational
    Isoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage.
  • Cellular localizationCytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGAP-2 antibody
    • Agap2 antibody
    • Agap2 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
    • AGAP2_HUMAN antibody
    • ANK repeat and PH domains 2 antibody
    • Arf GAP with GTP binding protein like antibody
    • Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2 antibody
    • Arf GAP with GTP-binding protein-like, ankyrin repeat and pleckstrin homology domains 2 antibody
    • Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 antibody
    • ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
    • centaurin gamma1 antibody
    • Centaurin-gamma-1 antibody
    • Centg1 antibody
    • cnt g1 antibody
    • Cnt-g1 antibody
    • Cnt2 antibody
    • FLJ16430 antibody
    • GGAP2 antibody
    • GTP binding and GTPase activating protein 2 antibody
    • GTP-binding and GTPase activating protein 2 antibody
    • GTP-binding and GTPase-activating protein 2 antibody
    • KIAA0167 antibody
    • mKIAA0167 antibody
    • Nuclear GTPase PIKE antibody
    • Phosphatidylinositol 3-kinase enhancer antibody
    • Phosphoinositide 3 kinase enhancer antibody
    • PI3K ENHANCER antibody
    • PIKE antibody
    see all

Anti-Centaurin gamma 1 antibody images

  • Lane 1 : Anti-Centaurin gamma 1 antibody (ab21271) at 0.5 µg/ml
    Lane 2 : Anti-Centaurin gamma 1 antibody (ab21271) at 1 µg/ml
    Lane 3 : Anti-Centaurin gamma 1 antibody (ab21271) at 2 µg/ml

    Lane 1 : Mouse brain cell lysate
    Lane 2 : Mouse brain cell lysate
    Lane 3 : Mouse brain cell lysate

    Predicted band size : 90 kDa
  • ab21271 at 10µg/ml staining Centaurin gamma 1 in mouse brain tissue by IHC

References for Anti-Centaurin gamma 1 antibody (ab21271)

ab21271 has not yet been referenced specifically in any publications.

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