Overview

  • Product nameAnti-Centaurin gamma 1 antibody
    See all Centaurin gamma 1 primary antibodies
  • Description
    Rabbit polyclonal to Centaurin gamma 1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A recombinant protein corresponding to amino acids 1-836 of human Centaurin gamma 1 protein.

  • Positive control
    • HEK293 cell lysates, untransfected or transfected with Centaurin gamma 1 recombinant protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab51796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 90 kDa.
ELISA 1/40000 - 1/200000.

Target

  • FunctionGTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
  • Tissue specificityIsoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart.
  • Sequence similaritiesBelongs to the centaurin gamma-like family.
    Contains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
  • DomainG domain binds GTP and has GTPase activity.
    Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
    Although both PH domains of isoforms 1 and 2 bind phospholipids, they differently regulate subcellular location. PH domain of isoform 1 directs the protein to the nucleus, but PH domain of isoform 2 directs it to the cytosol. PH domain of isoform 2 is required for binding to AP-1.
  • Post-translational
    modifications
    Isoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage.
  • Cellular localizationCytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGAP-2 antibody
    • Agap2 antibody
    • Agap2 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
    • AGAP2_HUMAN antibody
    • ANK repeat and PH domains 2 antibody
    • Arf GAP with GTP binding protein like antibody
    • Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2 antibody
    • Arf GAP with GTP-binding protein-like, ankyrin repeat and pleckstrin homology domains 2 antibody
    • Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 antibody
    • ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
    • centaurin gamma1 antibody
    • Centaurin-gamma-1 antibody
    • Centg1 antibody
    • cnt g1 antibody
    • Cnt-g1 antibody
    • Cnt2 antibody
    • FLJ16430 antibody
    • GGAP2 antibody
    • GTP binding and GTPase activating protein 2 antibody
    • GTP-binding and GTPase activating protein 2 antibody
    • GTP-binding and GTPase-activating protein 2 antibody
    • KIAA0167 antibody
    • mKIAA0167 antibody
    • Nuclear GTPase PIKE antibody
    • Phosphatidylinositol 3-kinase enhancer antibody
    • Phosphoinositide 3 kinase enhancer antibody
    • PI3K ENHANCER antibody
    • PIKE antibody
    see all

Anti-Centaurin gamma 1 antibody images

  • All lanes : Anti-Centaurin gamma 1 antibody (ab51796) at 1/10000 dilution

    Lane 1 : HEK293 cell lysate (empty vector)
    Lane 2 : HEK293 cell lysate transfected with Centaurin gamma 1 recombinant protein


    Predicted band size : 90 kDa
    Observed band size : 90 kDa

References for Anti-Centaurin gamma 1 antibody (ab51796)

ab51796 has not yet been referenced specifically in any publications.

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