Overview

  • Product nameAnti-CENTB1 antibody
    See all CENTB1 primary antibodies
  • Description
    Goat polyclonal to CENTB1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide: EKLSRRSHDLHTL, corresponding to C terminal amino acids 728-740 of Human CENTB1.

  • Positive control
    • Jurkat lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab15903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 80-90 kDa (predicted molecular weight: 85 kDa).Can be blocked with Human CENTB1 peptide (ab23280).

Target

  • FunctionGTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.
  • Tissue specificityHighest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
  • Sequence similaritiesContains 3 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 BAR domain.
    Contains 1 PH domain.
  • DomainPH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.
  • Post-translational
    modifications
    Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAP 1 antibody
    • ACAP1 antibody
    • ACAP1_HUMAN antibody
    • Arf GAP with coiled coil ANK repeat and PH domains 1 antibody
    • Arf GAP with coiled coil, ankyrin repeat, and pleckstrin homology domains 1 antibody
    • Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 antibody
    • ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 antibody
    • CENT B1 antibody
    • Centaurin beta 1 antibody
    • Centaurin-beta-1 antibody
    • CENTB 1 antibody
    • Cnt b1 antibody
    • Cnt-b1 antibody
    • Cntb1 antibody
    • KIAA0050 antibody
    see all

Anti-CENTB1 antibody images

  • Anti-CENTB1 antibody (ab15903) at 1 µg/ml + Jurkat cell lysate at 35 µg

    Predicted band size : 85 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

     

     

References for Anti-CENTB1 antibody (ab15903)

ab15903 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"