Overview

  • Product name
  • Description
    Goat polyclonal to CENTB1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:

    C-EKLSRRSHDLHTL

    , corresponding to C terminal amino acids 728-740 of Human CENTB1

  • Positive control
    • Jurkat lysate; Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab85945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notes
    ELISA: 1/32000.
    IHC-P: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 0.5 - 2 µg/ml for 1hr. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.
    • Tissue specificity
      Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
    • Sequence similarities
      Contains 3 ANK repeats.
      Contains 1 Arf-GAP domain.
      Contains 1 BAR domain.
      Contains 1 PH domain.
    • Domain
      PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.
    • Post-translational
      modifications
      Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAP 1 antibody
      • ACAP1 antibody
      • ACAP1_HUMAN antibody
      • Arf GAP with coiled coil ANK repeat and PH domains 1 antibody
      • Arf GAP with coiled coil, ankyrin repeat, and pleckstrin homology domains 1 antibody
      • Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 antibody
      • ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 antibody
      • CENT B1 antibody
      • Centaurin beta 1 antibody
      • Centaurin-beta-1 antibody
      • CENTB 1 antibody
      • Cnt b1 antibody
      • Cnt-b1 antibody
      • Cntb1 antibody
      • KIAA0050 antibody
      see all

    Images

    • ab85945 at 5µg/ml staining CENTB1 in human tonsil by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
    • Anti-CENTB1 antibody (ab85945) at 0.5 µg/ml + Jurkat lysate at 35 µg

      Predicted band size : 85 kDa
      Observed band size : 85 kDa

    References

    ab85945 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab85945.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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