Overview

  • Product nameAnti-CENTB1 antibody
    See all CENTB1 primary antibodies
  • Description
    Mouse polyclonal to CENTB1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human CENTB1, amino acids 1-740 (NP_055531.1)

  • Positive control
    • Human spleen tissue lysate, K562 cell lysate and CENTB1 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab89766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 82 kDa.

Target

  • FunctionGTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.
  • Tissue specificityHighest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
  • Sequence similaritiesContains 3 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 BAR domain.
    Contains 1 PH domain.
  • DomainPH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.
  • Post-translational
    modifications
    Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAP 1 antibody
    • ACAP1 antibody
    • ACAP1_HUMAN antibody
    • Arf GAP with coiled coil ANK repeat and PH domains 1 antibody
    • Arf GAP with coiled coil, ankyrin repeat, and pleckstrin homology domains 1 antibody
    • Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 antibody
    • ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 antibody
    • CENT B1 antibody
    • Centaurin beta 1 antibody
    • Centaurin-beta-1 antibody
    • CENTB 1 antibody
    • Cnt b1 antibody
    • Cnt-b1 antibody
    • Cntb1 antibody
    • KIAA0050 antibody
    see all

Anti-CENTB1 antibody images

  • Anti-CENTB1 antibody (ab89766) at 1 µg/ml + Human spleen tissue lysate at 50 µg

    Predicted band size : 82 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • Anti-CENTB1 antibody (ab89766) at 1 µg/ml + K562 cell lysate at 50 µg

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
    Additional bands at : 35 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-CENTB1 antibody (ab89766) at 1 µg/ml

    Lane 1 : CENTB1 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 82 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

References for Anti-CENTB1 antibody (ab89766)

ab89766 has not yet been referenced specifically in any publications.

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