Anti-CENTB1 (phospho S554) antibody (ab111378)


  • Product nameAnti-CENTB1 (phospho S554) antibody
    See all CENTB1 primary antibodies
  • Description
    Rabbit polyclonal to CENTB1 (phospho S554)
  • Specificityab111378 detects endogenous levels of CENTB1 only when phosphorylated at serine 554.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from Human CENTB1 around the phosphorylation site of serine 554 (P-G-SP-L-R).

  • Positive control
    • Human lung carcinoma tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 0.88% Sodium chloride, 50% Glycerol
    Note: without Mg2+ and Ca2+
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111378 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab111378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionGTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.
  • Tissue specificityHighest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
  • Sequence similaritiesContains 3 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 BAR domain.
    Contains 1 PH domain.
  • DomainPH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.
  • Post-translational
    Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAP 1 antibody
    • ACAP1 antibody
    • ACAP1_HUMAN antibody
    • Arf GAP with coiled coil ANK repeat and PH domains 1 antibody
    • Arf GAP with coiled coil, ankyrin repeat, and pleckstrin homology domains 1 antibody
    • Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 antibody
    • ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 antibody
    • CENT B1 antibody
    • Centaurin beta 1 antibody
    • Centaurin-beta-1 antibody
    • CENTB 1 antibody
    • Cnt b1 antibody
    • Cnt-b1 antibody
    • Cntb1 antibody
    • KIAA0050 antibody
    see all

Anti-CENTB1 (phospho S554) antibody images

  • ab111378 at 1/50 dilution staining CENTB1 in paraffin-embedded Human Lung carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

References for Anti-CENTB1 (phospho S554) antibody (ab111378)

ab111378 has not yet been referenced specifically in any publications.

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