Anti-CENTB1 (phospho S554) antibody (ab111378)

Overview

  • Product name
    Anti-CENTB1 (phospho S554) antibody
    See all CENTB1 primary antibodies
  • Description
    Rabbit polyclonal to CENTB1 (phospho S554)
  • Specificity
    ab111378 detects endogenous levels of CENTB1 only when phosphorylated at serine 554.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from Human CENTB1 around the phosphorylation site of serine 554 (P-G-SP-L-R).

  • Positive control
    • Human lung carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 0.88% Sodium chloride, 50% Glycerol
    Note: without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab111378 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.
  • Tissue specificity
    Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
  • Sequence similarities
    Contains 3 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 BAR domain.
    Contains 1 PH domain.
  • Domain
    PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.
  • Post-translational
    modifications
    Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAP 1 antibody
    • ACAP1 antibody
    • ACAP1_HUMAN antibody
    • Arf GAP with coiled coil ANK repeat and PH domains 1 antibody
    • Arf GAP with coiled coil, ankyrin repeat, and pleckstrin homology domains 1 antibody
    • Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 antibody
    • ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 antibody
    • CENT B1 antibody
    • Centaurin beta 1 antibody
    • Centaurin-beta-1 antibody
    • CENTB 1 antibody
    • Cnt b1 antibody
    • Cnt-b1 antibody
    • Cntb1 antibody
    • KIAA0050 antibody
    see all

Anti-CENTB1 (phospho S554) antibody images

  • ab111378 at 1/50 dilution staining CENTB1 in paraffin-embedded Human Lung carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

References for Anti-CENTB1 (phospho S554) antibody (ab111378)

ab111378 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab111378.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up