The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionGTPase-activating protein for the ADP ribosylation factor family (Potential). GTPase which may be involved in the degradation of expanded polyglutamine proteins through the ubiquitin-proteasome pathway.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the centaurin gamma-like family. Contains 3 ANK repeats. Contains 1 Arf-GAP domain. Contains 1 PH domain.
Cellular localizationCytoplasm. In cells upon oxidative stress or in brains of Machado-Joseph disease patients, translocates to PML nuclear bodies.
ab115459 at 5 µg/ml staining CENTG3 in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-CENTG3 antibody (ab115459)
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