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Rabbit polyclonal to Centlein
antigen sequence: LIEARKEVEV SQSKYNALSL QLSNKQTELI QKDMDITLVR KELQELQNLY KQNSTHTAQQ AELIQQLQVL NMDTQKVLRN QEDVHTAESI S corresponding to amino acids 289-379 of Human Centlein.
Human liver tissue; Human liver lysate; Human cell line U-251 MG
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.
Cytoplasm > cytoskeleton > centrosome > centriole. Colocalizes with gamma-tubulin during interphase and mitosis. Appears to associated with the mother centriole during G1 phase and with daughter centrioles towards G1/S phase.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Centlein antibody (ab122356)
ab122356, at 1/60 dilution, staining Centlein in paraffin-embedded Human liver tissue by Immunohistochemistry.
Western blot - Anti-Centlein antibody (ab122356)
All lanes : Anti-Centlein antibody (ab122356) at 1/250 dilution Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Immunocytochemistry/ Immunofluorescence - Anti-Centlein antibody (ab122356)
ab122356, at 4 µg/ml, staining Centlein in Human cell line U-251 MG by Immunofluorescence. Antibody staining is shown in green.
has not yet been referenced specifically in any publications.
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