Overview

  • Product nameAnti-Centrin 1 antibody
    See all Centrin 1 primary antibodies
  • Description
    Rabbit polyclonal to Centrin 1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Xenopus laevis, Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 157-172 of Human Centrin 1, with N-terminally added lysine. This sequence is highly conserved in centrin 1 of many species, e.g. mouse, drosophila, and Xenopus. It is also found in human and mouse centrin 2, but is not found in centrin 3.

  • Positive control
    • HeLa cell line.
  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 21 kDa. 1/2000, this concentration is recommended using a nuclear extract of the human epitheloid carcinoma HeLa cell line. Predicted molecular weight: 21 kDa.
ICC/IF 1/200. PubMed: 17299043

Suggested working dilution of 1/200 (see PMID: 17299043).

Methanol fixation may be preferable for this antibody based on publications and customer feedback.

Target

  • FunctionPlays a fundamental role in microtubule-organizing center structure and function.
  • Sequence similaritiesBelongs to the centrin family.
    Contains 4 EF-hand domains.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome. Centrosome of interphase and mitotic cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Caltractin antibody
    • Caltractin isoform 2 antibody
    • CEN 1 antibody
    • CEN1 antibody
    • Centrin EF hand protein 1 antibody
    • Centrin-1 antibody
    • Centrin1 antibody
    • CETN 1 antibody
    • CETN antibody
    • CETN1 antibody
    • CETN1_HUMAN antibody
    • EF hand protein 1 antibody
    see all

Anti-Centrin 1 antibody images

  • ab11257 staining Centrin 1 in mouse embryonic fibroblast cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with Ethanol (-200C) and blocking with 3% BSA/PBS  was done for 30 minutes at 220C. Samples were incubated with primary antibody (1/500) in in 3% BSA /0.1 % saponin/PBS for 1 hour at RT. An undiluted Alexa Fluor®546-conjugated donkey polyclonal to goat IgG (H&L) was used at dilution at 1/500 as secondary antibody. In the image, Green staining represents gamma tubulin, red - centrin.

    See Abreview

References for Anti-Centrin 1 antibody (ab11257)

This product has been referenced in:
  • Mukhopadhyay A  et al. 14-3-3? Prevents Centrosome Amplification and Neoplastic Progression. Sci Rep 6:26580 (2016). ICC/IF . Read more (PubMed: 27253419) »
  • Shiratsuchi G  et al. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34:97-114 (2015). Human . Read more (PubMed: 25385835) »

See all 13 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (gastrocoel roof plate)
Permeabilization Yes - 0.1% Triton X-100
Specification gastrocoel roof plate
Blocking step 3% BSA + 3% serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
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Verified customer

Submitted Sep 29 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Sample Mouse Cell (Liver)
Specification Liver
Permeabilization Yes - 0.5% Triton in PBS
Fixative Paraformaldehyde
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Submitted Aug 13 2014

Thank you for your response.

I am not sure what IB represents but assume the customer would mean either IP (Immunoprecipitation) or WB (Western blot) application? Is that correct?

I regret to inform you that I am not convinced that ...

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Thank you for your response.

Unfortunately, the two image files can't be opened so I am unable to take a look at the signal.

Tested and Guaranted Applications:

ab11257 - WB, ICC/IF

ab99337 - WB and IP - this has ...

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Thank you for your enquiry regarding our two products. I am very sorry to hear that you are having problems with this antibody.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on th...

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Thank you very much for your recent review of ab11257. Your review is now published on our website.
Since the results were below average, I'm contacting you to see if we can help resolve this problem. I would recommend reducing the amount of prote...

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Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 50 µg
Specification Hela
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Aug 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Specification Hela
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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Submitted Aug 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic stem)
Specification Embryonic stem
Fixative Methanol
Permeabilization Yes - 0.1% triton-X-100
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 25°C
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Prof. Reiter Lab

Verified customer

Submitted Apr 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse embryonic fibroblast (MEF))
Specification mouse embryonic fibroblast (MEF)
Fixative EtOH (-20C)
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
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Submitted Mar 22 2010

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"