Overview

  • Product name
  • Description
    Rabbit polyclonal to Centrin 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human, Xenopus laevis
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH, with a Cysteine residue linker corresponding to C terminal amino acids 155-168 of Human Centrin 1 (Uniprot ID: Q12798).

  • Positive control
    • HeLa and MCF7 crude cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab156858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 20 kDa.
ICC/IF 1/200 - 1/500.

Target

Images

  • ab156858 staining Centrin 1 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/1000. DAPI (Blue) was used as a nuclear counterstain.

  • All lanes : Anti-Centrin 1 antibody (ab156858) at 1/1000 dilution

    Lane 1 : HeLa cell lysate at 20 µg
    Lane 2 : MCF7 cell lysate at 20 µg
    Lane 3 : NIH3T3 cell lysate at 20 µg
    Lane 4 : Xenopus eggs cell lysate at 50 µg

    Predicted band size: 20 kDa

  • All lanes : Anti-Centrin 1 antibody (ab156858) at 1/1000 dilution

    Lane 1 : HeLa crude cell lysate at 10 µg
    Lane 2 : MCF7 crude cell lysate at 20 µg

    Predicted band size: 20 kDa

References

ab156858 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab156858.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up