Overview

  • Product nameAnti-Centrin 2 antibody
    See all Centrin 2 primary antibodies
  • Description
    Mouse monoclonal to Centrin 2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: NFGDFLTVMT QKMSEKDTKE EILKAFKLFD DDETGKISFK NLKRVAKELG ENLTDEELQE MIDEADRDGD GEVSEQEFLR IMKKTSLY, corresponding to amino acids 85-173 of Human Centrin 2

Properties

Applications

Our Abpromise guarantee covers the use of ab54525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested on recombinant protein, not endogenous samples.

Target

  • FunctionPlays a fundamental role in microtubule-organizing center structure and function. Required for centriole duplication and correct spindle formation. Has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CEP110.
    Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with RAD23B appears to stabilize XPC. In vitro, stimulates DNA binding of the XPC:RAD23B dimer.
    The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair.
  • Sequence similaritiesBelongs to the centrin family.
    Contains 4 EF-hand domains.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Nucleus. Centrosome of S-phase, interphase and mitotic cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 20kD calcium binding protein antibody
    • CALT antibody
    • caltractin antibody
    • Caltractin isoform 1 antibody
    • CEN2 antibody
    • centrin antibody
    • centrin, EF hand protein, 2 antibody
    • Centrin-2 antibody
    • Centrin2 antibody
    • CETN2 antibody
    • CETN2_HUMAN antibody
    • EF hand protein 2 antibody
    • EF-hand protein antibody
    see all

Anti-Centrin 2 antibody images

  • Western blot against tagged recombinant protein immunogen using ab54525 Centrin 2 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

References for Anti-Centrin 2 antibody (ab54525)

This product has been referenced in:
  • Ertych N  et al. Increased microtubule assembly rates influence chromosomal instability in colorectal cancer cells. Nat Cell Biol 16:779-91 (2014). Read more (PubMed: 24976383) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (RPE-1)
Permeabilization No
Specification RPE-1
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Methanol
Username

Quynh Nguyen

Verified customer

Submitted Apr 11 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Sample Mouse Cell (neuroblast)
Specification neuroblast
Permeabilization Yes - PBST
Fixative Methanol
Username

Abcam user community

Verified customer

Submitted May 31 2014

Thank you for your reply. I have compared the two sequences and they are 82% identical.  There is a small chance for cross reactivity and I will be happy to offer a testing discount code.  Please let me know if you are interested and I will send yo...

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Thank you for contacting Abcam regarding ab54525. Our testing discount program is one in which we ask customers to purchase an antibody and test it in an untested application and/or species.  After testing, we ask that you submit your results to us...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 23 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"