Anti-Centrin 2 with anti-GAPDH internal loading control antibody (ab80863)

Overview

• Product name
  Anti-Centrin 2 with anti-GAPDH internal loading control antibody
• Description
  Rabbit polyclonal to Centrin 2 with anti-GAPDH internal loading control
• Tested applications
  Suitable for: WBmore details
  Unsuitable for: ChIP,Flow Cyt,ICC/IF or IHC-P
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Chicken, Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Centrin 2 with anti-GAPDH internal loading control.

  Read Abcam's proprietary immunogen policy

• Positive control
  • This cocktail gave a positive signal in Jurkat whole cell lysate.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Spindle

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Cytokinesis

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Huntington's disease

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Isotype/Loading Controls
  • Loading Controls
  • GAPDH

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Nucl. Excision Repair

  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Calcium Binding Proteins

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Centrosome

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Metabolism
  • Types of disease
  • Cancer

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Plays a fundamental role in microtubule-organizing center structure and function. Required for centriole duplication and correct spindle formation. Has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CEP110.
  Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with RAD23B appears to stabilize XPC. In vitro, stimulates DNA binding of the XPC:RAD23B dimer.
  The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair.
• Sequence similarities
  Belongs to the centrin family.
  Contains 4 EF-hand domains.
• Cellular localization
  Cytoplasm > cytoskeleton > centrosome > centriole. Nucleus. Centrosome of S-phase, interphase and mitotic cells.

• Target information above from: UniProt accession P41208 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 1069 Human
  • Entrez Gene: 26370 Mouse
  • Entrez Gene: 84593 Rat
  • Omim: 300006 Human
  • SwissProt: Q2TBN3 Cow
  • SwissProt: P41208 Human
  • SwissProt: Q9R1K9 Mouse
  • Unigene: 82794 Human

  • Unigene: 24643 Mouse
  • Unigene: 232560 Rat

  see all
• Alternative names
  • CALT antibody
  • Caltractin (20kD calcium-binding protein) antibody
  • Caltractin isoform 1 antibody

  • CEN2 antibody
  • Centrin EF hand protein 2 antibody
  • Centrin-2 antibody
  • CETN2 antibody
  • CETN2_HUMAN antibody

  see all

Protocols

• Western blot protocols