Overview

  • Product nameAnti-Centrin 3 antibody
    See all Centrin 3 primary antibodies
  • Description
    Mouse monoclonal to Centrin 3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MSLALRSELV VDKTKRKKRR ELSEEQKQEI KDAFELFDTD KDEAIDYHEL KVAMRALGFD VKKADVLKIL KDYDREATGK ITFEDFNEVV TDWILERDPH , corresponding to amino acids 1-100 of Human Centrin 3

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a fundamental role in microtubule-organizing center structure and function.
    • Sequence similaritiesBelongs to the centrin family.
      Contains 4 EF-hand domains.
    • Cellular localizationCytoplasm > cytoskeleton > centrosome. Centrosome of interphase and mitotic cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDC31 homolog antibody
      • CEN 3 antibody
      • CEN3 antibody
      • Centrin EF hand protein 3 antibody
      • Centrin-3 antibody
      • Centrin3 antibody
      • CETN 3 antibody
      • Cetn3 antibody
      • CETN3_HUMAN antibody
      • EF hand superfamily member antibody
      • MGC12502 antibody
      • MGC138245 antibody
      see all

    Anti-Centrin 3 antibody images



    • Predicted band size : 20 kDa

    References for Anti-Centrin 3 antibody (ab54531)

    This product has been referenced in:
    • Burrell RA  et al. Replication stress links structural and numerical cancer chromosomal instability. Nature 494:492-6 (2013). Read more (PubMed: 23446422) »
    • Jani D  et al. Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export. Nucleic Acids Res 40:4562-73 (2012). WB . Read more (PubMed: 22307388) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Non-reduced Denaturing (11%)
    Sample Human Cell lysate - whole cell (293T)
    Specification 293T
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 14 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"