The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/500. It appears from the reference Babcock et al that it is not necessary to perform antigen retrieval.
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Babcock KK et al. A synaptic membrane glycine-, glutamate- and thienylcyclohexylpiperidine-binding protein: isolation and immunochemical characterization. Neurochem Int29:507-19 (1996).
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